IgE binding conformational epitopes of Asp f 3, a major allergen of Aspergillus fumigatus
H Ramachandran, V Jayaraman, B Banerjee… - Clinical …, 2002 - Elsevier
sp f 3 has been identified as one of the major allergens of Aspergillus fumigatus associated
with the sensitization and immune responses in allergic bronchopulmonary aspergillosis
(ABPA). In order to understand the structure/function relationship of Asp f 3, we studied
synthetic peptides and constructed mutants deleted of specific IgE binding regions. The
mutated allergens were obtained by expressing the genes and studied by ELISA for their
reactivity with IgE from patients with ABPA. Seven linear IgE binding regions spanning the …
with the sensitization and immune responses in allergic bronchopulmonary aspergillosis
(ABPA). In order to understand the structure/function relationship of Asp f 3, we studied
synthetic peptides and constructed mutants deleted of specific IgE binding regions. The
mutated allergens were obtained by expressing the genes and studied by ELISA for their
reactivity with IgE from patients with ABPA. Seven linear IgE binding regions spanning the …
