Thiopental-induced insulin secretion via activation of IP3-sensitive calcium stores in rat pancreatic β-cells

HQ Dou, YF Xu, JP Sun, S Shang… - … of Physiology-Cell …, 2012 - journals.physiology.org
HQ Dou, YF Xu, JP Sun, S Shang, S Guo, LH Zheng, C Chen, IC Bruce, X Yu, Z Zhou
American Journal of Physiology-Cell Physiology, 2012journals.physiology.org
While glucose-stimulated insulin secretion depends on Ca2+ influx through voltage-gated
Ca2+ channels in the cell membrane of the pancreatic β-cell, there is also ample evidence
for an important role of intracellular Ca2+ stores in insulin secretion, particularly in relation to
drug stimuli. We report here that thiopental, a common anesthetic agent, triggers insulin
secretion from the intact pancreas and primary cultured rat pancreatic β-cells. We
investigated the underlying mechanisms by measurements of whole cell K+ and Ca2+ …
While glucose-stimulated insulin secretion depends on Ca2+ influx through voltage-gated Ca2+ channels in the cell membrane of the pancreatic β-cell, there is also ample evidence for an important role of intracellular Ca2+ stores in insulin secretion, particularly in relation to drug stimuli. We report here that thiopental, a common anesthetic agent, triggers insulin secretion from the intact pancreas and primary cultured rat pancreatic β-cells. We investigated the underlying mechanisms by measurements of whole cell K+ and Ca2+ currents, membrane potential, cytoplasmic Ca2+ concentration ([Ca2+]i), and membrane capacitance. Thiopental-induced insulin secretion was first detected by enzyme-linked immunoassay, then further assessed by membrane capacitance measurement, which revealed kinetics distinct from glucose-induced insulin secretion. The thiopental-induced secretion was independent of cell membrane depolarization and closure of ATP-sensitive potassium (KATP) channels. However, accompanied by the insulin secretion stimulated by thiopental, we recorded a significant intracellular [Ca2+] increase that was not from Ca2+ influx across the cell membrane, but from intracellular Ca2+ stores. The thiopental-induced [Ca2+]i rise in β-cells was sensitive to thapsigargin, a blocker of the endoplasmic reticulum Ca2+ pump, as well as to heparin (0.1 mg/ml) and 2-aminoethoxydiphenyl borate (2-APB; 100 μM), drugs that inhibit inositol 1,4,5-trisphosphate (IP3) binding to the IP3 receptor, and to U-73122, a phospholipase C inhibitor, but insensitive to ryanodine. Thapsigargin also diminished thiopental-induced insulin secretion. Thus, we conclude that thiopental-induced insulin secretion is mediated by activation of the intracellular IP3-sensitive Ca2+ store.
American Physiological Society