miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARγ expression

SY Kim, AY Kim, HW Lee, YH Son, GY Lee… - Biochemical and …, 2010 - Elsevier
SY Kim, AY Kim, HW Lee, YH Son, GY Lee, JW Lee, YS Lee, JB Kim
Biochemical and biophysical research communications, 2010Elsevier
microRNAs (miRNAs) are non-coding small RNAs regulating gene expression, cell growth,
and differentiation. Although several miRNAs have been implicated in cell growth and
differentiation, it is barely understood their roles in adipocyte differentiation. In the present
study, we reveal that miR-27a is involved in adipocyte differentiation by binding to the
PPARγ 3′-UTR whose sequence motifs are highly conserved in mammals. During
adipogenesis, the expression level of miR-27a was inversely correlated with that of …
microRNAs (miRNAs) are non-coding small RNAs regulating gene expression, cell growth, and differentiation. Although several miRNAs have been implicated in cell growth and differentiation, it is barely understood their roles in adipocyte differentiation. In the present study, we reveal that miR-27a is involved in adipocyte differentiation by binding to the PPARγ 3′-UTR whose sequence motifs are highly conserved in mammals. During adipogenesis, the expression level of miR-27a was inversely correlated with that of adipogenic marker genes such as PPARγ and adiponectin. In white adipose tissue, miR-27a was more abundantly expressed in stromal vascular cell fraction than in mature adipocytes fraction. Ectopic expression of miR-27a in 3T3-L1 pre-adipocytes repressed adipocyte differentiation by reducing PPARγ expression. Interestingly, the level of miR-27a in mature adipocyte fraction of obese mice was down-regulated than that of lean mice. Together, these results suggest that miR-27a would suppress adipocyte differentiation through targeting PPARγ and thereby down-regulation of miR-27a might be associated with adipose tissue dysregulation in obesity.
Elsevier