Using Tandem Mass Spectrometry to Quantify Site‐Specific Chlorination and Nitration of Proteins: Model System Studies with High‐Density Lipoprotein Oxidized by …
B Shao, JW Heinecke - Methods in enzymology, 2008 - Elsevier
Protein oxidation is implicated in atherogenesis and other inflammatory conditions.
Measuring levels of chlorinated and nitrated proteins in biological matrices serves as a
quantitative index of oxidative stress in vivo. One potential mechanism for oxidative stress
involves myeloperoxidase, a heme protein expressed by neutrophils, monocytes, and some
populations of macrophages. The enzyme uses hydrogen peroxide to generate an array of
cytotoxic oxidants, including hypochlorous acid (HOCl), a potent chlorinating intermediate …
Measuring levels of chlorinated and nitrated proteins in biological matrices serves as a
quantitative index of oxidative stress in vivo. One potential mechanism for oxidative stress
involves myeloperoxidase, a heme protein expressed by neutrophils, monocytes, and some
populations of macrophages. The enzyme uses hydrogen peroxide to generate an array of
cytotoxic oxidants, including hypochlorous acid (HOCl), a potent chlorinating intermediate …