[HTML][HTML] Myeloperoxidase targets apolipoprotein AI, the major high density lipoprotein protein, for site-specific oxidation in human atherosclerotic lesions
B Shao, S Pennathur, JW Heinecke - Journal of Biological Chemistry, 2012 - ASBMB
Oxidative damage by myeloperoxidase (MPO) has been proposed to deprive HDL of its
cardioprotective effects. In vitro studies reveal that MPO chlorinates and nitrates specific
tyrosine residues of apoA-I, the major HDL protein. After Tyr-192 is chlorinated, apoA-I is
less able to promote cholesterol efflux by the ABCA1 pathway. To investigate the potential
role of this pathway in vivo, we used tandem mass spectrometry with selected reaction
monitoring to quantify the regiospecific oxidation of apoA-I. This approach demonstrated that …
cardioprotective effects. In vitro studies reveal that MPO chlorinates and nitrates specific
tyrosine residues of apoA-I, the major HDL protein. After Tyr-192 is chlorinated, apoA-I is
less able to promote cholesterol efflux by the ABCA1 pathway. To investigate the potential
role of this pathway in vivo, we used tandem mass spectrometry with selected reaction
monitoring to quantify the regiospecific oxidation of apoA-I. This approach demonstrated that …