[HTML][HTML] Tracing Hepatic Gluconeogenesis Relative to Citric Acid Cycle Activity in Vitro and in Vivo: COMPARISONS IN THE USE OF [3-13C] LACTATE,[2-13C] …
M Beylot, MV Soloviev, F David, BR Landau… - Journal of Biological …, 1995 - ASBMB
The validity of the use of a carbon tracer for investigating liver intermediary metabolism in
vivo requires that the labeling pattern of liver metabolites not be influenced by metabolism of
the tracer in other tissues. To identify such specific tracer, livers from 48-h starved rats were
perfused with recirculating buffer containing [3-13 C] lactate,[2-13 C] acetate, or α-keto [3-13
C] isocaproate. Conscious 48-h starved rats were infused with the same tracers for 5 h. The
labeling patterns of liver glutamate and extracellular glucose were assayed by gas …
vivo requires that the labeling pattern of liver metabolites not be influenced by metabolism of
the tracer in other tissues. To identify such specific tracer, livers from 48-h starved rats were
perfused with recirculating buffer containing [3-13 C] lactate,[2-13 C] acetate, or α-keto [3-13
C] isocaproate. Conscious 48-h starved rats were infused with the same tracers for 5 h. The
labeling patterns of liver glutamate and extracellular glucose were assayed by gas …