CRISPR RNA–guided activation of endogenous human genes
Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease
(dCas9) to repress endogenous genes in bacteria and human cells. Here we show that
single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain
to increase expression of endogenous human genes. This proof-of-principle work shows
that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can
target heterologous effector domains to endogenous sites in human cells.
(dCas9) to repress endogenous genes in bacteria and human cells. Here we show that
single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain
to increase expression of endogenous human genes. This proof-of-principle work shows
that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can
target heterologous effector domains to endogenous sites in human cells.
Abstract
Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
nature.com