[HTML][HTML] Calcium dependence of polycystin-2 channel activity is modulated by phosphorylation at Ser812
Y Cai, G Anyatonwu, D Okuhara, KB Lee, Z Yu… - Journal of Biological …, 2004 - ASBMB
Polycystin-2 (PC-2) is a non-selective cation channel that, when mutated, results in
autosomal dominant polycystic kidney disease. In an effort to understand the regulation of
this channel, we investigated the role of protein phosphorylation in PC-2 function. We
demonstrated the direct incorporation of phosphate into PC-2 in cells and tissues and found
that this constitutive phosphorylation occurs at Ser 812, a putative casein kinase II (CK2)
substrate domain. Ser 812 can be phosphorylated by CK2 in vitro and substitution S812A …
autosomal dominant polycystic kidney disease. In an effort to understand the regulation of
this channel, we investigated the role of protein phosphorylation in PC-2 function. We
demonstrated the direct incorporation of phosphate into PC-2 in cells and tissues and found
that this constitutive phosphorylation occurs at Ser 812, a putative casein kinase II (CK2)
substrate domain. Ser 812 can be phosphorylated by CK2 in vitro and substitution S812A …