[HTML][HTML] Differential phosphorylation of RhoGDI mediates the distinct cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion
Z Wang, DC Thurmond - Journal of biological chemistry, 2010 - ASBMB
Cdc42 cycling through GTP/GDP states is critical for its function in the second/granule
mobilization phase of insulin granule exocytosis in pancreatic islet beta cells, although the
identities of the Cdc42 cycling proteins involved remain incomplete. Using a tandem affinity
purification-based mass spectrometry screen for Cdc42 cycling factors in beta cells, RhoGDI
was identified. RNA interference-mediated depletion of RhoGDI from isolated islets
selectively amplified the second phase of insulin release, consistent with the role of RhoGDI …
mobilization phase of insulin granule exocytosis in pancreatic islet beta cells, although the
identities of the Cdc42 cycling proteins involved remain incomplete. Using a tandem affinity
purification-based mass spectrometry screen for Cdc42 cycling factors in beta cells, RhoGDI
was identified. RNA interference-mediated depletion of RhoGDI from isolated islets
selectively amplified the second phase of insulin release, consistent with the role of RhoGDI …