In response to cold, norepinephrine (NE)-induced triacylglycerol hydrolysis (lipolysis) in
adipocytes of brown adipose tissue (BAT) provides fatty acid substrates to mitochondria for
heat generation (adaptive thermogenesis). NE-induced lipolysis is mediated by protein
kinase A (PKA)-dependent phosphorylation of perilipin, a lipid droplet-associated protein
that is the major regulator of lipolysis. We investigated the role of perilipin PKA
phosphorylation in BAT NE-stimulated thermogenesis using a novel mouse model in which …