[HTML][HTML] Analysis of lipolytic protein trafficking and interactions in adipocytes
JG Granneman, HPH Moore, RL Granneman… - Journal of Biological …, 2007 - ASBMB
This work examined the colocalization, trafficking, and interactions of key proteins involved
in lipolysis during brief cAMP-dependent protein kinase A (PKA) activation. Double label
immunofluorescence analysis of 3T3-L1 adipocytes indicated that PKA activation increases
the translocation of hormonesensitive lipase (HSL) to perilipin A (Plin)-containing droplets
and increases the colocalization of adipose tissue triglyceride lipase (Atgl) with its
coactivator, Abhd5. Imaging of live 3T3-L1 preadipocytes transfected with Aquorea victoria …
in lipolysis during brief cAMP-dependent protein kinase A (PKA) activation. Double label
immunofluorescence analysis of 3T3-L1 adipocytes indicated that PKA activation increases
the translocation of hormonesensitive lipase (HSL) to perilipin A (Plin)-containing droplets
and increases the colocalization of adipose tissue triglyceride lipase (Atgl) with its
coactivator, Abhd5. Imaging of live 3T3-L1 preadipocytes transfected with Aquorea victoria …