[HTML][HTML] Modulation of hormone-sensitive lipase and protein kinase A-mediated lipolysis by perilipin A in an adenoviral reconstituted system
SC Souza, KV Muliro, L Liscum, P Lien… - Journal of Biological …, 2002 - ASBMB
Perilipin (Peri) A is a phosphoprotein located at the surface of intracellular lipid droplets in
adipocytes. Activation of cyclic AMP-dependent protein kinase (PKA) results in the
phosphorylation of Peri A and hormone-sensitive lipase (HSL), the predominant lipase in
adipocytes, with concurrent stimulation of adipocyte lipolysis. To investigate the relative
contributions of Peri A and HSL in basal and PKA-mediated lipolysis, we utilized NIH 3T3
fibroblasts lacking Peri A and HSL but stably overexpressing acyl-CoA synthetase 1 (ACS1) …
adipocytes. Activation of cyclic AMP-dependent protein kinase (PKA) results in the
phosphorylation of Peri A and hormone-sensitive lipase (HSL), the predominant lipase in
adipocytes, with concurrent stimulation of adipocyte lipolysis. To investigate the relative
contributions of Peri A and HSL in basal and PKA-mediated lipolysis, we utilized NIH 3T3
fibroblasts lacking Peri A and HSL but stably overexpressing acyl-CoA synthetase 1 (ACS1) …