In situ hybridization (ISH) is a widely applied technique used for visualizing specific nucleic
acid sequences at chromosomal, cytologic, and histologic levels. It sometimes fails,
however, to demonstrate precise cell identity, early stages of gene expression and variants
of alternative splicing because of its limited resolution. To overcome this shortcoming, we
have developed an improved ISH technique at the electron microscopic (EM) level by
conducting en bloc hybridization before embedding (pre-embedding) and immuno-EM …