1, 25-dihydroxycholecalciferol: a potent stimulator of bone resorption in tissue culture
LG Raisz, CL Trummel, MF Holick, HF DeLuca - Science, 1972 - science.org
LG Raisz, CL Trummel, MF Holick, HF DeLuca
Science, 1972•science.org1, 25-Dihydroxycholecalciferol (DHCC), isolated from kidney homogenates incubated with
25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated 45Ca
from fetal rat bones in organ culture, at concentrations of 10-10 to 10-8 M. The dose
response curves for 1, 25-DHCC and 25-HCC, the parent compound, are parallel, but 1, 25-
DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of
either agent leads to prolonged bone resorption.
25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated 45Ca
from fetal rat bones in organ culture, at concentrations of 10-10 to 10-8 M. The dose
response curves for 1, 25-DHCC and 25-HCC, the parent compound, are parallel, but 1, 25-
DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of
either agent leads to prolonged bone resorption.
1,25-Dihydroxycholecalciferol (DHCC), isolated from kidney homogenates incubated with 25-hydroxycholecalciferol (HCC), stimulated the release of previously incorporated 45Ca from fetal rat bones in organ culture, at concentrations of 10-10 to 10-8M. The dose response curves for 1,25-DHCC and 25-HCC, the parent compound, are parallel, but 1,25-DHCC is about 100 times as potent on a weight basis. Brief exposure to maximum doses of either agent leads to prolonged bone resorption.
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