Age‐related accumulation of LFA‐1high cells in a CD8+CD45RAhigh T cell population
M Okumura, Y Fujii, Y Takeuchi, K Inada… - European journal of …, 1993 - Wiley Online Library
M Okumura, Y Fujii, Y Takeuchi, K Inada, K Nakahara, H Matsuda
European journal of immunology, 1993•Wiley Online LibraryThe differential expression of CD45 isoforms has been suggested as a marker for stages of
post‐thymic T cell development, that is, CD45RA+ CD45R0− T cells and CD45RA−
CD45R0+ T cells are supposed to be virgin and memory cells respectively. Recently,
several adhesion molecules have been shown to be up‐regulated on the cell surface of
memory T cells, and have been suggested to serve as a memory marker. In this study, we
investigated the levels of LFA‐1 expression on T cells in various subpopulations defined by …
post‐thymic T cell development, that is, CD45RA+ CD45R0− T cells and CD45RA−
CD45R0+ T cells are supposed to be virgin and memory cells respectively. Recently,
several adhesion molecules have been shown to be up‐regulated on the cell surface of
memory T cells, and have been suggested to serve as a memory marker. In this study, we
investigated the levels of LFA‐1 expression on T cells in various subpopulations defined by …
Abstract
The differential expression of CD45 isoforms has been suggested as a marker for stages of post‐thymic T cell development, that is, CD45RA+CD45R0− T cells and CD45RA−CD45R0+ T cells are supposed to be virgin and memory cells respectively. Recently, several adhesion molecules have been shown to be up‐regulated on the cell surface of memory T cells, and have been suggested to serve as a memory marker. In this study, we investigated the levels of LFA‐1 expression on T cells in various subpopulations defined by CD45 isoform expression in donors of various ages.
In CD4+ T cells, the proportion of LFA‐1high cells among CD45RAhighCD45R0‐T cells remained low in all age groups and did not show significant accumulation with age. CD4+CD45RA−CD45R0highTcells expressed LFA‐1 at a higher level than CD4+CD45RAhighCD45R0− T cells. Thus, the currently prevailing view that CD45RA and CD45R0 can be markers for virgin and primed cells was consistent with LFA‐1 expression in CD4+ T cell population.
In CD8+ T cells, however, CD45RAhighCD45R0− T cells consisted of two distinct subpopulations, LFA‐1low and LFA‐1high cells, whereas CD45RA−CD45R0high T cells were almost exclusively LFA‐1high When CD29 expression was examined in place of LFA‐1 expression, similar results were obtained; CD45RAhigh CD45R0− T cells consisted of two distinct subpopulations, CD29‐to low and CD29high cells, while CD45RA‐CD45R0high T cells were mostly CD29high.
The proportion of LFA‐1high cells in the CD8+CD45RAhigh T cell subpopulation increased significantly as a function of age (r = 0.9, p < 0.001). It ranged from 8% in a 14‐year‐old donor to 94% in a 79‐year‐old donor. Furthermore, the proportion of CD8+CD45RAhighLFA‐1high cells in the CD8+ T cell population increased significantly as a function of age (r = 0.85, p < 0.001). On the other hand, the proportion of LEA‐1high cells in CD8+CD45RA− T cell subpopulation exceeded 90% in most donors irrespective of age.
These results indicate that the CD8+CD45RAhighCD45R0− T cell subpopulation contains a considerable number of LFA‐1high cells and CD29high cells, phenotypically similar to previously activated cells. Thus, in terms of LFA‐1 and CD29 expressions, the simple scheme that CD45RA is a marker of virgin cells is not applicable to the CD8+ T cell population.
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