[HTML][HTML] DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
Nedd8 activates ubiquitination by increasing the efficiency of polyubiquitin chain assembly
through its covalent conjugation to cullin molecules. Here we report the isolation, cloning,
and characterization of a novel human Nedd8-specific protease called DEN1. Human DEN1
is encoded by AAH31411. 1, a previously uncharacterized protein of 212 amino acids that
shares homology with the Ulp1 cysteinyl SUMO deconjugating enzyme family. Recombinant
human DEN1, purified from bacteria, selectively binds to Nedd8 and hydrolyzes C-terminal …
through its covalent conjugation to cullin molecules. Here we report the isolation, cloning,
and characterization of a novel human Nedd8-specific protease called DEN1. Human DEN1
is encoded by AAH31411. 1, a previously uncharacterized protein of 212 amino acids that
shares homology with the Ulp1 cysteinyl SUMO deconjugating enzyme family. Recombinant
human DEN1, purified from bacteria, selectively binds to Nedd8 and hydrolyzes C-terminal …