[HTML][HTML] Identification and characterization of DEN1, a deneddylase of the ULP family
T Gan-Erdene, K Nagamalleswari, L Yin, K Wu… - Journal of Biological …, 2003 - ASBMB
To identify deneddylases, proteases with specificity for hydrolysis of Nedd8 derivatives, a
facile method was developed for the synthesis of Nedd8 amidomethylcoumarin (a substrate)
and Nedd8 vinyl sulfone (an inhibitor). Deneddylase activity is necessary to reverse the
conjugation of Nedd8 to cullin, a modification that regulates at least some ubiquitin ligases.
The reaction of Nedd8 vinyl sulfone with LM (TK–) mouse fibroblast lysates identified two
deneddylases. The deubiquitinating enzyme UCH-L3 is labeled by both ubiquitin vinyl …
facile method was developed for the synthesis of Nedd8 amidomethylcoumarin (a substrate)
and Nedd8 vinyl sulfone (an inhibitor). Deneddylase activity is necessary to reverse the
conjugation of Nedd8 to cullin, a modification that regulates at least some ubiquitin ligases.
The reaction of Nedd8 vinyl sulfone with LM (TK–) mouse fibroblast lysates identified two
deneddylases. The deubiquitinating enzyme UCH-L3 is labeled by both ubiquitin vinyl …