[HTML][HTML] The NEDD8 pathway is essential for SCFβ-TrCP-mediated ubiquitination and processing of the NF-κB precursor p105
RE Amir, K Iwai, A Ciechanover - Journal of Biological Chemistry, 2002 - ASBMB
The p50 subunit of NF-κB is generated by limited processing of the precursor p105. IκB
kinase-mediated phosphorylation of the C-terminal domain of p105 recruits the SCF β-TrCP
ubiquitin ligase, resulting in rapid ubiquitination and subsequent processing/degradation of
p105. NEDD8 is known to activate SCF ligases following modification of their cullin
component. Here we show that NEDDylation is required for conjugation and processing of
p105 by SCF β-TrCP following phosphorylation of the molecule. In a crude extract, a …
kinase-mediated phosphorylation of the C-terminal domain of p105 recruits the SCF β-TrCP
ubiquitin ligase, resulting in rapid ubiquitination and subsequent processing/degradation of
p105. NEDD8 is known to activate SCF ligases following modification of their cullin
component. Here we show that NEDDylation is required for conjugation and processing of
p105 by SCF β-TrCP following phosphorylation of the molecule. In a crude extract, a …