A dataset of human liver proteins identified by protein profiling via isotope-coded affinity tag (ICAT) and tandem mass spectrometry
Proteins from human liver carcinoma Huh7 cells, representing transformed liver cells, and
cultured primary human fetal hepatocytes (HFH) and human HH4 hepatocytes, representing
nontransformed liver cells, were extracted and processed for proteome analysis. Proteins
from stimulated cells (interferon-α treatment for the Huh7 and HFH cells and induction of
hepatitis C virus [HCV] proteins for the HH4 cells) and corresponding control cells were
labeled with light and heavy cleavable ICAT reagents, respectively. The labeled samples …
cultured primary human fetal hepatocytes (HFH) and human HH4 hepatocytes, representing
nontransformed liver cells, were extracted and processed for proteome analysis. Proteins
from stimulated cells (interferon-α treatment for the Huh7 and HFH cells and induction of
hepatitis C virus [HCV] proteins for the HH4 cells) and corresponding control cells were
labeled with light and heavy cleavable ICAT reagents, respectively. The labeled samples …
