Saturation kinetics of palmitate uptake in perfused skeletal muscle
LP Turcotte, B Kiens, EA Richter - FEBS letters, 1991 - Elsevier
LP Turcotte, B Kiens, EA Richter
FEBS letters, 1991•ElsevierWe investigated the kinetics of palmitate uptake in a physiological skeletal muscle
preparation by using the isolated perfused rat hindquarter. When plotted against the
unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis-
Menten relation with a calculated V max and K m of 16.3 nmol· min− 1· g− 1 and 0.06 μmol·
l− 1, respectively. These results show that, as in isolated cell systems, uptake of free fatty
acids in perfused skeletal muscle follows saturation kinetics consistent with carrier-mediated …
preparation by using the isolated perfused rat hindquarter. When plotted against the
unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis-
Menten relation with a calculated V max and K m of 16.3 nmol· min− 1· g− 1 and 0.06 μmol·
l− 1, respectively. These results show that, as in isolated cell systems, uptake of free fatty
acids in perfused skeletal muscle follows saturation kinetics consistent with carrier-mediated …
Abstract
We investigated the kinetics of palmitate uptake in a physiological skeletal muscle preparation by using the isolated perfused rat hindquarter. When plotted against the unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis-Menten relation with a calculated Vmax and Km of 16.3 nmol · min−1 · g−1 and 0.06 μmol·l−1, respectively. These results show that, as in isolated cell systems, uptake of free fatty acids in perfused skeletal muscle follows saturation kinetics consistent with carrier-mediated membrane transport of free fatty acids.
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