SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigen
G Chu, PA Sharp - Gene, 1981 - Elsevier
G Chu, PA Sharp
Gene, 1981•ElsevierA modification of the Graham and Van der Eb (1973) DNA-calcium phosphate
coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA.
The transfection is done in suspension after detachment of cells by trypsin digestion.
Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen
by indirect immunofluorescence and by assaying for the presence of SV40 early message
by the Berk and Sharp (1978) technique.
coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA.
The transfection is done in suspension after detachment of cells by trypsin digestion.
Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen
by indirect immunofluorescence and by assaying for the presence of SV40 early message
by the Berk and Sharp (1978) technique.
Abstract
A modification of the Graham and Van der Eb (1973) DNA-calcium phosphate coprecipitation technique is shown to routinely transfect 15% of CV-1 cells with SV40 DNA. The transfection is done in suspension after detachment of cells by trypsin digestion. Transfection efficiency was measured by staining cells for the presence of SV40 T-antigen by indirect immunofluorescence and by assaying for the presence of SV40 early message by the Berk and Sharp (1978) technique.
Elsevier
