Role of N-linked glycosylation in rat renal Na/Pi-cotransport.
G Hayes, A Busch, M Lötscher, S Waldegger… - Journal of Biological …, 1994 - ASBMB
Our laboratory recently identified a sodium-dependent transport system for phosphate from
rat kidney cortex (NaPi-2; Magagnin, S., Werner, A., Markovich, D., Sorribas, V., Stange, G.,
Biber, J., and Murer, H.(1993) Proc. Natl. Acad. Sci. USA 90, 5979-5983). In the present
study we have investigated whether or not this cotransporter is glycosylated and the role of
N-glycosylation in determining its function. Glycosidase digestion of the NaPi-2 protein from
rat brush border membranes, in vitro translation studies, or oocyte expression of the NaPi-2 …
rat kidney cortex (NaPi-2; Magagnin, S., Werner, A., Markovich, D., Sorribas, V., Stange, G.,
Biber, J., and Murer, H.(1993) Proc. Natl. Acad. Sci. USA 90, 5979-5983). In the present
study we have investigated whether or not this cotransporter is glycosylated and the role of
N-glycosylation in determining its function. Glycosidase digestion of the NaPi-2 protein from
rat brush border membranes, in vitro translation studies, or oocyte expression of the NaPi-2 …
