The doxycycline-inducible reverse tetracycline transactivator (rtTA) is frequently used to
overexpress transgenes in a temporally regulated fashion in vivo. These systems are,
however, often limited by the levels of transgene expression in the absence of dox
administration. The tetracycline-controlled transcriptional silencer (tTS), a fusion protein
containing thetet repressor and the KRAB-AB domain of the kid-1 transcriptional repressor,
is inhibited by doxycycline. We hypothesized that tTS would tighten control of transgene …