Real-time quantitative RT–PCR after laser-assisted cell picking
L Fink, W Seeger, L Ermert, J Hänze, U Stahl… - Nature medicine, 1998 - nature.com
Nature medicine, 1998•nature.com
NEW TECHNOLOGY one11. However, affecting factors will vary only negligibly among
single samples so that K is assumed to be equal and thus does not influence the
comparison of calculated relative ratios. For comparison of different runs the run efficiencies
have to be calculated. The efficiency of PCR provides information about the amplification
rate and varies from 0 to 1. One (= 100%) means that in each cycle the amount of copies is
doubled. A serial dilution of a PCR product of PBGD in concentrations corresponding to …
single samples so that K is assumed to be equal and thus does not influence the
comparison of calculated relative ratios. For comparison of different runs the run efficiencies
have to be calculated. The efficiency of PCR provides information about the amplification
rate and varies from 0 to 1. One (= 100%) means that in each cycle the amount of copies is
doubled. A serial dilution of a PCR product of PBGD in concentrations corresponding to …
NEW TECHNOLOGY one11. However, affecting factors will vary only negligibly among single samples so that K is assumed to be equal and thus does not influence the comparison of calculated relative ratios. For comparison of different runs the run efficiencies have to be calculated. The efficiency of PCR provides information about the amplification rate and varies from 0 to 1. One (= 100%) means that in each cycle the amount of copies is doubled. A serial dilution of a PCR product of PBGD in concentrations corresponding to copy numbers of picked cells served for generating a standard curve. The slope of this standard curve is required for calculation of the run efficiency. The relation is given by:
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