A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine model
R Sloane, JCS de Azavedo, JP Arbuthnott… - FEMS microbiology …, 1991 - academic.oup.com
R Sloane, JCS de Azavedo, JP Arbuthnott, PJ Hartigan, B Kreiswirth, R Novick, TJ Foster
FEMS microbiology letters, 1991•academic.oup.comThe gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic
replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-
enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This
suggests that TSST-1 is the only determinant produced by this strain that can induce the
symptoms of shock in rabbits. A novel method for allelic replacement involving transduction
of plasmid integrants is described.
replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-
enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This
suggests that TSST-1 is the only determinant produced by this strain that can induce the
symptoms of shock in rabbits. A novel method for allelic replacement involving transduction
of plasmid integrants is described.
Summary
The gene coding for toxic shock syndrome toxin-1 in S. aureus was inactivated by allelic replacement in two TSS-associated strains. One mutant derived from FRI1169 (a non-enterotoxigenic strain) lacked virulence in the rabbit uterine chamber infection model. This suggests that TSST-1 is the only determinant produced by this strain that can induce the symptoms of shock in rabbits. A novel method for allelic replacement involving transduction of plasmid integrants is described.
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