Jalaluddin Sheikh, John R. Czeczulin, Susan Harrington, Susan Hicks, Ian R. Henderson, Chantal Le Bouguénec, Pierre Gounon, Alan Phillips, James P. Nataro
Penetration of 042 and 042aap through mucus. Columns of 10 mg/ml mucin in HBSS were prepared in tuberculin syringes, and the bacterial inocula were applied to the top of the column. The columns were incubated at 37°C for 75 minutes. After this time, the mucin suspension was drained from the column in 0.1-ml fractions, and the number of 042 (black bars) or 042aap (white bars) was determined by quantitative plating in triplicate. Bars represent SD. Fraction 10 represents the bottom of the column, fraction 1 the top. *P < 0.05 by Student’s t test of log-transformed data.