The number of cytokine-producing cells per million PBMCs in response to whole recombinant Der p 1. The gray bars represent atopic symptomatic patients (S), the black bars represent the atopic asymptomatic patients (AA), and the white bars represent the nonatopic individuals (NA). (a) Data for cytokines IL-4, IL-5, IL-13, and IL-10. (b) Data for cytokines IFN-γ and TNF-α. (c and d) Unstimulated PBMCs and recombinant Der p 1–stimulated PBMCs, respectively (gated on CD8+ T cells). The y axis shows staining with anti-CD69, and the x axis shows staining with anti–IFN-γ. (d) 0.1% of CD8+ T cells produced IFN-γ in response to whole recombinant Der p 1 staining.